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Image Search Results
Journal: Hypertension
Article Title: Novel Role of Rac1/WAVE Signaling Mechanism in Regulation of the Epithelial Na + Channel
doi: 10.1161/hypertensionaha.110.157784
Figure Lengend Snippet: Figure 4. Expression profiles of N-Wiskott-Aldrich syndrome protein (WASP)/WAVEs in the rat kidney tissue and in epithelial cells. A, Expression profiles of N-WASP/WAVEs in principal cells. Figure shows Western blot analysis of endogenous N-WASP/WAVEs in mouse principal cells (mpkCCDc14 and M-1). Western blot analysis was repeated 4 times with similar results. Left lane for WAVE1 represents a control for cell lysate derived from 3T3/A31 cell line. B, Representative immunohistochemical staining for N-WASP and WAVE1 detection in kidney cortical sections of Sprague-Dawley rats at 60 magnification. Negative control tissue (control) stained with secondary antibodies in the absence of primary antibodies is also shown. Cortical collecting ducts (CCD) are shown by arrows.
Article Snippet: The mammalian expression vectors encoding mouse -, -, and -ENaC have been described previously.15,16 The expression vectors encoding Wiskott-Aldrich syndrome-like (SC111092), WAS protein family,
Techniques: Expressing, Western Blot, Control, Derivative Assay, Immunohistochemical staining, Staining, Negative Control